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Name: Leung Kin Man University number: 2009109720 Date of experimentation: 25-2-2010 BIOL1133: biologic Science Laboratory Course Experiment 4: Expression of humans secretin sensory receptor concretion protein in E coli. Aim: The suggest of the experiment is to express human secretin receptor fusion protein in E coli by conducting SDS-PAGE gel electrophoresis and estimate the molecular size of that protein. Abstract: In this experiment, the human secretin receptor fusion protein was expressed exploitation the SDS-PAGE technique. The pre-induced and the sample subsequently IPTG induction was use to extract the human secretin receptor protein by exploitation denaturing buffer zone and elution buffer. The molecular weight of the protein feces also be estimated by comparing the bands on the protein ladder. foot: Massive production of active protein is usually done by recombinant deoxyribonucleic acid technology. E coli. was employ as vector for introm ission and expression of fusion protein. E coli. is a germ-negative bacteria with stunt man membrane layer and gamin plasmid. In this experiment, the N-terminal extracellular arena of the human secretin receptor in E coli. was expressed using IPTG inducible system.

Secretin is the key hormone that prudent for the regulation of gastrointestinal choke by regulating the secretion of bicarbonate and enzyme from the exocrine pancreas. This quite a little help to neutralize stomachic acid (hydrochloric acid) and hence substantiate an optimum pH care for for intestinal digestion. The action of secretin is in the m ain controlled by high-affinity membrane rec! eptors distributed in our body and it is tissue-specific. The N-terminal coding component part of the extracellular domain of the receptor protein is obtained by conducting polymerase chain reaction (PCR) mutagenesis method. This technique was achieved by using specific deoxyribonucleic acid primers and DNA strand as a template to start the PCR reaction. The coding sequence was introduced into the bacterial DNA as a carrier of the coding sequence. Upon IPTG...If you want to posture a full essay, put in it on our website: BestEssayCheap.com

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